Semi-Targeted Ultra-High-Performance Chromatography Coupled to Mass Spectrometry Analysis of Phenolic Metabolites in Plasma of Elderly Adults
A group of 23 elderly persons was given functional meals (a beverage and a muffin) specially formulated for the prevention of sarcopenia (age-related loss of muscle mass). Plasma samples were taken at the beginning of the intervention and after 30 days of consuming the functional meals. A semi-ta...
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| 其他作者: | , , , |
| 格式: | Artículo |
| 语言: | en_US |
| 出版: |
2022
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| 主题: | |
| 在线阅读: | https://doi.org/10.3791/63164 https://www.jove.com/es/t/63164/semi-targeted-ultra-high-performance-chromatography-coupled-to-mass |
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| 总结: | A group of 23 elderly persons was given functional meals (a beverage and a muffin)
specially formulated for the prevention of sarcopenia (age-related loss of muscle
mass). Plasma samples were taken at the beginning of the intervention and after
30 days of consuming the functional meals. A semi-targeted ultra-high-performance
chromatography coupled with tandem mass (UPLC-MS/MS) analysis was carried
out to identify phenolic compounds and their metabolites. Plasma proteins were
precipitated with ethanol and the samples were concentrated and resuspended
in the mobile phase (1:1 acetonitrile: water) before injection into the UPLC-MS/
MS instrument. Separation was carried out with a C18 reverse-phase column,
and compounds were identified using their experimental mass, isotopic distribution,
and fragment pattern. Compounds of interest were compared to those of data
banks and the internal semi-targeted library. Preliminary results showed that the
major metabolites identified after the intervention were phenylacetic acid, glycitin, 3-
hydroxyphenylvaleric acid, and gomisin M2. |
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