Comparison of three sampling procedures for evaluating intestinal villi: A swine model¤
Background: Villi morphology and function affect the absorption capacity of the small intestine. Most tissues are fragile and their morphology may change with excessive manipulation and inadequate sampling techniques. Intestinal sampling includes methodologies such as cutting longitudinally or tra...
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| 格式: | Artículo |
| 语言: | en_US |
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2018
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| 在线阅读: | https://doi.org/10.17533/udea.rccp.v31n1a01 https://aprendeenlinea.udea.edu.co/revistas/index.php/rccp/article/view/328833 |
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| 总结: | Background: Villi morphology and function affect the absorption capacity of the small intestine. Most
tissues are fragile and their morphology may change with excessive manipulation and inadequate sampling
techniques. Intestinal sampling includes methodologies such as cutting longitudinally or transversely,
keeping the intestinal content in it and preserving all in a 10% formalin solution; washing the intestinal
sample in saline solution while emptying it by pressing downwards with two fingers, conserving the
sample in a 10% formalin solution and knotting both ends of the sample, introducing 10% formalin into
it and preserving it in the same solution. Objective: To compare height, area and desquamation caused
by washing, pressing, and knotting used in sampling and conservation techniques of small intestine villi
of pigs. Methods: Samples (n = 270) from duodenum, jejunum and ileum of 30 Landrace × Yorkshire
crossed pigs, aged 7 to 8 months were randomly subjected to washing, soft pressing or knotting procedures,
fixed in 10% formalin solution, embedded in paraffin, and stained with eosin and hematoxylin. Intestinal
villi in each slide were observed to determine height, surface area and cellular desquamation of each
villus. Results: Villi height from duodenum and ileum knotted samples was higher (p<0.05) compared
with samples from the other procedures in the same anatomical portion, which were similar to each
other (p>0.05). Villi from knotted jejunum samples were the shortest (p<0.05) compared to the other
two procedures, which were similar to each other (p>0.05). Knotted samples from ileum had larger villi
area compared with the rest of the procedures and intestinal portions (p<0.05). Villi desquamation was similar among procedures and portions of the intestine (p>0.05). Conclusion: Knotting is the recommended
procedure for intestinal cell morphometry evaluation, as values of villi height and area are higher. Desquamation
in the three procedures may be related to epithelial restoration processes. |
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