Effect of Moringa oleifera seed extract on antimicrobial activity and in vitro fertilization ability of cryopreserved ram semen
Cryopreservation has adverse effects on the post-thaw sperm quality due to oxidative stress and the presence of bacteria. To minimize such effects, plant extracts have been included in the composition of the semen diluents. The objective of the present study was to evaluate the antimicrobial and...
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| Other Authors: | , , , , , , |
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| Format: | Artículo |
| Language: | English |
| Published: |
2022
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| Subjects: | |
| Online Access: | https://doi.org/10.1111/rda.14233 https://onlinelibrary.wiley.com/doi/full/10.1111/rda.14233 https://pubmed.ncbi.nlm.nih.gov/35997503/ |
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| Summary: | Cryopreservation has adverse effects on the post-thaw
sperm quality due to oxidative
stress and the presence of bacteria. To minimize such effects, plant extracts
have been included in the composition of the semen diluents. The objective of the
present study was to evaluate the antimicrobial and antioxidant effects of Moringa
oleifera seed extract (MOSE) on cryopreserved ram semen, as well as its impact on in
vitro fertilization. Semen from six hair rams was treated with five treatments before
cryopreservation: Control (without any antibiotic), Standard (conventional antibiotic),
1.0, 10.0, and 50.0 mg/ml of MOSE. Post-thawing
sperm characteristics were evaluated
by the computer-assisted
semen analysis. Antimicrobial activity was assessed
by counting colony-forming
units (CFU) and the antioxidant capacity by the ferric
reducing antioxidant power method. A heterologous in vitro fertilization technique
was implemented to measure the fertilization rate. Progressive and rapid motility,
membrane and acrosome integrity, and active mitochondria were higher (p < .05) in
the 10.0 mg/ml treatment compared with Standard after thawing. All M. oleifera treatments
showed inhibition of CFU. The antioxidant capacity of M. oleifera seed extract
was higher in the 10.0 and 50.0 mg/ml treatments. Fertilization rate (cleavage percentage)
was higher (p < .05) in the 10.0 mg/ml (82.9 ± 10.0) and Control (82.5 ± 9.9)
treatments compared with Standard (73.7 ± 9.1). The addition of 10.0 mg/ml of MOSE
to ram semen inhibits the development of microorganisms and improves sperm characteristics
and the in vitro fertility of the semen. |
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