Assessment on In Vitro Probiotic Attributes of Lactobacillus plantarum Isolated From Horse Feces.
This study was designed to assess in vitro probiotic attributes of potent bacterium isolated from the feces of healthy horse. Initially, a total of eight bacteria were isolated from the feces and evaluated their an tibacterial activities against indicator bacterial pathogens using agar well diffusi...
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| Other Authors: | , , , , , , , |
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| Format: | Artículo |
| Language: | en_US |
| Published: |
2021
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| Subjects: | |
| Online Access: | https://doi.org/10.1016/j.jevs.2021.103769 https://www.j-evs.com |
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| Summary: | This study was designed to assess in vitro probiotic attributes of potent bacterium isolated from the feces
of healthy horse. Initially, a total of eight bacteria were isolated from the feces and evaluated their an tibacterial activities against indicator bacterial pathogens using agar well diffusion assay. Results showed
significant (P < .05) antibacterial property of Lactobacillus plantarum strain LF4 against pathogens tested
with maximum growth inhibitory activity of 320.16 ± 3.4 AU/mL against Staphylococcus aureus. Further, in
vitro probiotic properties of strain LF4 were determined using standard methodologies. Strain LF4 main tained its viability towards acidic condition (pH 2.0) and simulated gastric juice (pH 2.0) with total cell
counts of 1.6 ± 0.18 and 1.7 ± 0.18 log cfu/mL, respectively. Moreover, the strain was observed resistant
to oxgall (0.5% w/v) up to 36 hours. The isolate showed significant (P < .05) hydrophobicity property
(60.3 ± 1.6%), auto-aggregation trait (41.31 ± 1.5%), and moderate proteolytic activity. Strain LF4 revealed
significant (P < .05) rate of DPPH scavenging (15.3 ± 1.3-69.7 ± 1.3%) and hydroxyl radical scavenging
(11.3 ± 1.3 to 56.4 ± 1.3%) in a concentration dependent manner. Additionally, the isolate was observed
susceptible to all the conventional antibiotics tested, thereby indicating its safer utilization. In conclusion,
findings suggested the colossal applications of L. plantarum strain LF4 as an ideal probiotic bacterium in
equine industries. |
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