Biointeraction of Erythrocyte Ghost Membranes with Gold Nanoparticles Fluorescents
The application of new technologies for treatments against different diseases is increasingly innovative and effective. In the case of nanomedicine, the combination of nanoparticles with biological membranes consists of a “camouflage” technique, which improves biological interaction and minimizes...
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| Main Author: | |
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| Other Authors: | , , , , |
| Format: | Artículo |
| Language: | en_US |
| Published: |
2021
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| Subjects: | |
| Online Access: | https://doi.org/10.3390/ma14216390 https://www.mdpi.com/1996-1944/14/21/6390 |
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| Summary: | The application of new technologies for treatments against different diseases is increasingly
innovative and effective. In the case of nanomedicine, the combination of nanoparticles with
biological membranes consists of a “camouflage” technique, which improves biological interaction
and minimizes the secondary effects caused by these remedies. In this work, gold nanoparticles synthesized
by chemical reduction (Turkevich 13 nm) were conjugated with fluorescein isothiocyanate
to amplify their optical properties. Fluorescent nanoparticles were deposited onto the surface of
hemoglobin-free erythrocytes. Ghost erythrocytes were obtained from red blood cells by density
gradient separation in a hypotonic medium and characterized with fluorescence, optical, and electron
microscopy; the average size of erythrocyte ghosts was 9 m. Results show that the functional groups
of sodium citrate (COO-) and fluorophore (-N=C=S) adhere by electrostatic attraction to the surface
of the hemoglobin-free erythrocyte membrane, forming the membrane–particle–fluorophore. These
interactions can contribute to imaging applications, by increasing the sensitivity of measurement
caused by surface plasmon resonance and fluorescence, in the context of biological membranes. |
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