Influencia del Ca2+, pH, agar y reguladores de crecimiento en la propagación in vitro de Echinocactus parryi (Engelm)

Echinocactus parryi is a threatened and endemic species of the municipality of Juarez, Chihuahua, Mexico that presents important limitations for its propagation. The objective of this study was to evaluate the effect of growth regulators, calcium concentration (Ca2+), environmental pH and gelli...

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Bibliographic Details
Main Author: Osuna Avila, Pedro
Other Authors: García González, Dolores Adilene, Flores Margez, Juan Pedro, Santos Diaz, Maria del Socorro
Format: Artículo
Language:spa
Published: 2020
Subjects:
BAP
AIA
Online Access:https://doi.org/10.28940/terra.v38i3.734
https://www.terralatinoamericana.org.mx/index.php/terra/article/view/734
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Summary:Echinocactus parryi is a threatened and endemic species of the municipality of Juarez, Chihuahua, Mexico that presents important limitations for its propagation. The objective of this study was to evaluate the effect of growth regulators, calcium concentration (Ca2+), environmental pH and gelling concentration in the morphogenetic response of E. parryi. The seeds were germinated in Murashige and Skoog (MS) medium at 25% of the concentration of their salts (¼ MS). Epicotyls were cultured in an inverted position in media with different concentrations of 6-benzylaminopurine (BAP) (0-10 mg L-1), indole-3- acetic acid (AIA) (0-0.5 mg L-1), pH 5.7 and 8, Ca2+ (13.2 µM and 26.4 µM) and agar (7 and 10 g L-1). The highest sprout formation (2.9 sprouts per explant) was obtained in MS medium with 2 mg L-1 of BAP combined with 0.5 mg L-1 of AIA, 13.2 µM of Ca2+, pH 8, and 7 g L-1 of agar, at 120 days of culture. The sprouts were transferred to MS medium with 2,3,5 tri‑iodobenzoic acid (TIBA) (0, 0.5, 1 and 2 mg L-1) to induce root formation. The highest rooting (70.5%) was obtained in medium with 0.5 mg L-1 of TIBA. The survival of the plants was 58% at 5 months. This is the f irst report that describes the in vitro regeneration of E. parryi and constitutes an important advance for its propagation and conservation.